Subgroup analysis of DD-CKD patients across prespecified baseline ESA dose* subgroups1

Subgroup analyses1:

The ESA baseline dose analyses presented within are part of a subgroup analysis of the pivotal INNO2VATE clinical trials that has been previously published. The subgroup analyses were not powered to show differences between treatment arms and include certain subgroups with small sample sizes.

The subgroup analyses presented here have not been published previously except in abstract form and are not contained in the Vafseo product labeling.

Data from the two Phase 3 pivotal trials based on baseline ESA dose: low dose (less than or equal to 90 U/kg/wk), intermediate dose (greater than 90 U/kg/wk and less than 300 U/kg/wk), and high dose (greater than or equal to 300 U/kg/wk). The trials compared Vafseo to darbepoetin alfa for efficacy (mean change in Hb from baseline to the primary evaluation period of Weeks 24-36 and secondary evaluation period of Weeks 40-52) and safety (MACE).*,† Patients were randomized 1:1 to receive Vafseo with a starting dose of 300 mg once daily or darbepoetin alfa per prescribing information. Across the two trials, 1770 patients on Vafseo were converted from an ESA at baseline (low dose, n=916; intermediate dose, n=724; and high dose, n=102).

There were also 1759 patients on darbepoetin alfa who were converted from an ESA at baseline (low dose, n=968; intermediate dose, n=693; and high dose, n=98). Dosing in each treatment arm followed the protocol-specified dose-adjustment algorithm to achieve and sustain target Hb levels within 10 to 11 g/dL (US) or 10 to 12 g/dL (outside the US).

Data limitations1:

  • No multiplicity adjustments were applied for MACE or efficacy analyses in the subgroups
  • All analyses are presented for descriptive purposes only and should be interpreted with caution
  • A higher proportion of patients in the high ESA dose subgroup were Black and had lower hemoglobin concentrations, weights, and BMIs, which could have influenced the results
*ESA doses were converted to IV epoetin equivalent unit per kilogram per week (U/kg/wk): darbepoetin alfa to IV epoetin was 1:200; methoxy polyethylene glycol-epoetin beta to IV epoetin was 1:220; SC epoetin to IV epoetin was 1:1.25.1
MACE was defined as all-cause mortality, nonfatal myocardial infarction, and nonfatal stroke.2
BMI=body mass index; CKD=chronic kidney disease; DD-CKD=dialysis-dependent chronic kidney disease; ESA=erythropoiesis-stimulating agent; Hb=hemoglobin; IV=intravenous; MACE=major adverse cardiovascular event; SC=subcutaneous.

Prevalent Dialysis Trial (INNO2VATE-2) subgroup analysis: Efficacy stratified by baseline ESA dose

Treatment with Vafseo helped patients maintain baseline Hb levels during the PEP and SEP regardless of baseline ESA dose1

Patients treated with Vafseo in the Prevalent Dialysis Trial (INNO2VATE-2) (N=1777) were categorized into prespecified subgroups based on their baseline ESA dose1:

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Mean change in Hb with Vafseo by baseline ESA dose5 Overall
(N=1777)
Low Dose
≤90 U/kg/wk
(n=916)
Intermediate Dose
>90 to <300 U/kg/wk
(n=724)
High Dose
≥300 U/kg/wk
(n=102)
Mean baseline Hb concentration, g/dL mean±SD 10.25 ± 0.85 10.33 ± 0.81 10.22 ± 0.87 9.72 ± 0.91
Weeks 24-36 (PEP)
Mean baseline Hb concentration (observed + imputed), g/dL mean±SD 10.36 ± 1.01 10.53 ± 0.99 10.24 ± 0.98 9.78 ± 0.95
LS mean change in Hb from baseline (95% CI) 0.19 (0.12, 0.25) 0.25 (0.17, 0.33) 0.12 (0.02, 0.23) -0.11 (-0.53, 0.30)
Weeks 40‐52 (SEP)
Mean baseline Hb concentration (observed + imputed), g/dL mean±SD 10.40 ± 1.04 10.60 ± 1.05 10.23 ± 0.99 9.81 ± 0.91
LS mean change in Hb from baseline (95% CI) 0.23 (0.16, 0.29) 0.33 (0.24, 0.41) 0.10 (-0.01, 0.21) 0.08 (-0.35, 0.51)

CI=confidence interval; ESA=erythropoiesis-stimulating agent; Hb=hemoglobin; LS=least squares; PEP=primary evaluation period; SD=standard deviation; SEP=secondary evaluation period; wk=week.

Hb concentration in Vafseo and darbepoetin alfa treatment arms over time by ESA dose1,2

  • In patients treated with Vafseo, there was a transient decline in mean Hb levels in the high baseline ESA dose subgroup during weeks 2-8, after which levels increased by weeks 24–36. A less pronounced transient decline was observed in the intermediate baseline ESA dose subgroup but not in the low baseline ESA subgroup1
Line charts depicting the mean hemoglobin levels over time in 3 subgroups stratified by baseline ESA dose, showing responses of patients receiving Vafseo compared with darbepoetin alfa

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Data outside the primary (Weeks 24-36) and secondary (Weeks 40-52) evaluation periods are descriptive.

These graphs display mean Hb levels over time. Means ±SD (denoted by I bars) are presented here to show the extent of variability, which might have been less apparent if means ±SE were presented, given the large sample size.

Target Hb ranges were 10 to 11 g/dL in the US and 10 to 12 g/dL outside the US.2
  ESA=erythropoiesis-stimulating agent; Hb=hemoglobin; SD=standard deviation; SE=standard error; wk=week.

Prevalent Dialysis Trial (INNO2VATE-2) subgroup analysis: Safety

Cardiovascular outcomes in the prespecified subgroup analysis1,3,§

The incidence of MACE|| among patients treated with Vafseo was similar across baseline ESA doses.1,3

Forest plot depicting the incidence of major adverse cardiovascular events (MACE) by baseline ESA dose for patients receiving Vafseo compared with darbepoetin alfa

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The data are being presented for descriptive purposes only and should be interpreted with caution.
§ Darbepoetin alfa was titrated according to product Prescribing Information.2
|| MACE was defined as all-cause mortality, nonfatal myocardial infarction, and nonfatal stroke.2
Excluding vascular access thrombosis.3
  CI=confidence interval; ESA=erythropoiesis-stimulating agent; HR=hazard ratio; MACE=major adverse cardiovascular event; wk=week.

Treatment-emergent serious adverse events of special interest (≥10%)
by baseline ESA dose subgroups3

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  ≤90 U/kg/wk >90 to <300 U/kg/wk ≥300 U/kg/wk
Event, n (%) Vafseo
(n=949)
Darbepoetin Alfa
(n=992)
Vafseo
(n=764)
Darbepoetin Alfa
(n=738)
Vafseo
(n=111)
Darbepoetin Alfa
(n=103)
Any TE SAESI 337
(35.5)
396
(39.9)
312
(40.8)
338
(45.8)
48
(43.2)
57
(55.3)
Hypertension 122
(12.9)
165
(16.6)
144
(18.8)
159
(21.5)
25
(22.5)
35
(34.0)
Congestive Heart Failure 77
(8.1)
97
(9.8)
85
(11.1)
92
(12.5)
14
(12.6)
17
(16.5)
Hyperkalemia 70
(7.4)
98
(9.9)
75
(9.8)
84
(11.4)
15
(13.5)
21
(20.4)
Hepatotoxicity 60
(6.3)
59
(5.9)
53
(6.9)
64
(8.7)
14
(12.6)
10
(9.7)

ESA=erythropoiesis-stimulating agent; TE SAESI=treatment-emergent serious adverse events of special interest; wk=week.

Dosing in the Prevalent Dialysis Trial (INNO2VATE-2)

Vafseo doses over time across subgroups by baseline ESA dose††

  • All patients started on Vafseo 300 mg at study entry regardless of baseline ESA dose1
  • In the Vafseo treatment arm, the protocol allowed dose increases of 150 mg every 4 weeks. Dose decreases could occur more frequently. Doses may range from 150 mg to 600 mg1
  • By week 12, 52.3%, 64%, and 71.8% of patients were increased to 450 mg or 600 mg in the low, intermediate, and high baseline ESA dose subgroups, respectively1
    -

    In the high baseline group, 50% of patients were receiving 600 mg

  • During PEP and SEP, these percentages increased further, especially in the high baseline ESA dose subgroup1
Bar charts depicting dosing for patients per week within 3 subgroups based on ESA dose

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This analysis includes only those patients who were on Vafseo and not those whose dosages were interrupted due to elevated hemoglobin levels, ESA rescue, or adverse events. Data presented are the most common administered dose received during that study week. Data are observational and doses may have been increased or decreased per protocol.1

††Omits percentage of patients on 0 mg.1

ESA=erythropoiesis-stimulating agent; PEP=primary evaluation period; SEP=secondary evaluation period; wk=week.

Learn more about Vafseo PI dosing and dosing data in the overall study population